References

We list some of the key references we have used in this Protein Science Webpage Assignment below.

All links were last assessed on 31 May 2011.

Key Reference

Crystalstructure of QscR, a Pseudomonas aeruginosa quorum sensing signal receptor.[pdf]

Lintz MJ, Oinuma K, Wysoczynski CL, Greenberg EP, Churchill ME.

Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15763-8. Epub 2011 Sep 12.

Other References

1. Qin Y, Su S, Farrand SK (2007) Molecular basis of transcriptional antiactivation. TraM disrupts the TraR-DNA complex through stepwise interactions. J Biol Chem 282:19979–19991.
2. Seet Q, Zhang LH (2011) Anti-activator QslA defines the quorum sensing threshold and response in Pseudomonas aeruginosa. Mol Microbiol 80:951–965.
3. Stevens AM, Queneau Y, Soulere L, von Bodman S, Doutheau A (2011) Mechanisms and synthetic modulators of AHL-dependent gene regulation. Chem Rev 111:4–27.
4. Chen G, et al. (2011) A strategy for antagonizing quorum sensing. Mol Cell 42:199–209.
5. Circular Dichroism Spectroscopy Tutorial
6. Teresa R. de Kievit and Barbara H. Iglewski Bacterial Quorum Sensing in Pathogenic Relationships Infect. Immun. September 2000 68:4839-4849; doi:10.1128/IAI.68.9.4839-4849.2000
7. Clay Fuqua (2007) GUEST COMMENTARY:
The QscR Quorum-Sensing Regulon of Pseudomonas aeruginosa: an Orphan Claims Its Identity J. Bacteriol. May 1, 2006 188:3169-3171; doi:10.1128/JB.188.9.3169-3171.2006

Tools

1. http://www.pdb.org/pdb/home/home.do
   Link to RCSB Protein Data Bank for crystal structures of proteins studied (with associated ligands).
2. PyMOL Molecular Visualisation software
3. Paint (used for editing pictures)

Acknowledgement

We are grateful to the Protein Science Course Convenor, Prof Stephen Curry, for his guidance and instructions on this coursework. We would like to extend our gratitude to the Life Science Undergraduate Office for sending emails and reminders regarding this coursework. Also, we would like to thank our coursemates who commented constructively on our blog to help us improve. Last but not least, we hope our blog assessor will be pleased by our blog presentation and the effort we have put into this coursework. 

Cheers,

Terrence and Stalo

2nd Year Biochemists

Imperial College London